metamorph tracking Search Results


track objects function of the metamorph image analysis software  (MetaMorph Inc)
90
MetaMorph Inc track objects function of the metamorph image analysis software
Track Objects Function Of The Metamorph Image Analysis Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc03894203-44-23-28?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
track objects function of the metamorph image analysis software - by Bioz Stars, 2026-07
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track points function  (MetaMorph Inc)
90
MetaMorph Inc track points function
Track Points Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pm35594862-243-19-23?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
track points function - by Bioz Stars, 2026-07
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metamorph tracking plugin  (MetaMorph Inc)
90
MetaMorph Inc metamorph tracking plugin
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Metamorph Tracking Plugin, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc07865731-286-7-6?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
metamorph tracking plugin - by Bioz Stars, 2026-07
90/100 stars
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track objects module  (MetaMorph Inc)
90
MetaMorph Inc track objects module
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Track Objects Module, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc03944332-409-9-13?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
track objects module - by Bioz Stars, 2026-07
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trackobj particle-tracking algorithm  (MetaMorph Inc)
90
MetaMorph Inc trackobj particle-tracking algorithm
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Trackobj Particle Tracking Algorithm, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc02814791-81-14-18?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
trackobj particle-tracking algorithm - by Bioz Stars, 2026-07
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template recognition-based tracking tool of  (MetaMorph Inc)
90
MetaMorph Inc template recognition-based tracking tool of
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Template Recognition Based Tracking Tool Of, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc02172664-222-11-14?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
template recognition-based tracking tool of - by Bioz Stars, 2026-07
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offline software track-point  (MetaMorph Inc)
90
MetaMorph Inc offline software track-point
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Offline Software Track Point, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/us10794907-808-34-34?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
offline software track-point - by Bioz Stars, 2026-07
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time-lapse tracking feature of  (MetaMorph Inc)
90
MetaMorph Inc time-lapse tracking feature of
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Time Lapse Tracking Feature Of, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/10__1523_slash_jneurosci__21___24___09655__2001-117-1-5?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
time-lapse tracking feature of - by Bioz Stars, 2026-07
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6.1 cell tracking module  (MetaMorph Inc)
90
MetaMorph Inc 6.1 cell tracking module
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
6.1 Cell Tracking Module, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc02928007-190-24-23?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
6.1 cell tracking module - by Bioz Stars, 2026-07
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track points application  (MetaMorph Inc)
90
MetaMorph Inc track points application
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Track Points Application, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc03172168-226-19-23?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
track points application - by Bioz Stars, 2026-07
90/100 stars
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track object software  (MetaMorph Inc)
90
MetaMorph Inc track object software
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Track Object Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc02987444-383-9-15?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
track object software - by Bioz Stars, 2026-07
90/100 stars
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tracking methods  (MetaMorph Inc)
90
MetaMorph Inc tracking methods
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Tracking Methods, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/pmc03809019-300-39-44?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
tracking methods - by Bioz Stars, 2026-07
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Image Search Results


SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

Journal: International Journal of Molecular Sciences

Article Title: Glutamine Uptake via SNAT6 and Caveolin Regulates Glutamine–Glutamate Cycle

doi: 10.3390/ijms22031167

Figure Lengend Snippet: SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

Article Snippet: Tracking experiments were performed using a Metamorph tracking plugin with pixel size cut off = 5.

Techniques: Expressing, Microscopy, Fluorescence, Labeling, Derivative Assay, Transfection, Sequencing